sdaAA [2020-08-04 09:25:41]

L-serine deaminase

locus: BSU_15860

pI: 4.87

mw: 30.78 kDa

protein length: 300 aa

gene length: 903 bp

function: serine utilization

product: L-serine deaminase (alpha chain)

essential: no

ec: 4.3.1.17

synonyms: ylpA,sdaA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]

Gene

Coordinates: 1,658,930 1,659,832

Phenotypes of a mutant

• delayed [SW|biofilm formation] [pubmed|31138626]

The protein

Catalyzed reaction/ biological activity

• L-serine --> NH₄⁺ + pyruvate (according to UniProt)

Protein family

• iron-sulfur dependent L-serine dehydratase family (with [protein|BC19D5B3764503298ED7136FC9F63A68F6AC464F|SdaAB], according to UniProt)

[SW|Cofactors]

• Fe-S cluster [Pubmed|22686449]

Expression and Regulation

Operons

• genes: [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]-[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15860 ([gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACTGGCTCCTCCTGAC, downstream forward: _UP4_ATTTTCGGAGGAGCGCTAGG
• BKK15860 ([gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACTGGCTCCTCCTGAC, downstream forward: _UP4_ATTTTCGGAGGAGCGCTAGG

References

• 22383849,22686449,24161940,31138626,32743959