argB [2019-07-31 09:03:40]

N-acetylglutamate 5-phosphotransferase

locus: BSU_11210

pI: 6.21

mw: 27.57 kDa

protein length: 258 aa

gene length: 777 bp

function: biosynthesis of arginine

product: N-acetylglutamate 5-phosphotransferase

essential: no

ec: 2.7.2.8

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]

Gene

Coordinates: 1,197,326 1,198,102

The protein

Catalyzed reaction/ biological activity

• ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate (according to Swiss-Prot)

Protein family

• acetylglutamate kinase family (single member, according to UniProt)

Structure

• [PDB|2BTY] (from Thermotoga maritima, 45% identity) [pubmed|16376937]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]-[gene|0FF858E44B3C557EE2C8B3749A458F152E1C535C|argJ]-[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]-[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]-[gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]-[gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]-[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]
  description: [Pubmed|7511775]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7511775], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC]: repression, [Pubmed|1312212], in [regulon|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24843172], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]

  regulation

  • repressed by arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC]) [Pubmed|1312212]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

Biological materials

Mutant

• GP2481 (''[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]''::''ermC''), available in [SW|Jörg Stülke]'s lab
• BKE11210 (''[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]''::''ermC'') (available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE11210 ([gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATTTCGCTCCCCT, downstream forward: _UP4_GTCAAAGCAAAGGAGGCTGT
• BKK11210 ([gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATTTCGCTCCCCT, downstream forward: _UP4_GTCAAAGCAAAGGAGGCTGT
• GP2856 (''[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

Expression vectors

• pGP3010: expression of Strep-''argB'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
• pGP3011: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
• pGP3012: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

• 6096675,24843172,12107147,16376937,1312212,7511775