yfjP

similar to DNA-3-methyladenine glycosidase II

Locus: BSU_08010

Isoelectric point: 5.52

Molecular weight: 33.03 kDa

Protein length: 287 aa

Gene length: 864 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 3.2.2.21

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination/ based on similarity

Gene

Coordinates: 872,425 873,288

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to UniProt)

Protein family

• alkylbase DNA glycosidase AlkA family (with AlkA, according to UniProt)

Structure

• 2H56 (from B. halodurans, corresponds to aa 119 ... 285, 29% identity)

Expression and Regulation

Operons

• Genes: yfjP-yfjO
  Description: PubMed

Biological materials

Mutant

• MGNA-C275 (yfjP::erm), available at the NBRP B. subtilis, Japan
• BKE08010 (yfjP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGATCCACCCTTTTTT, downstream forward: _UP4_TAATTCGTCTGACAATGAAG
• BKK08010 (yfjP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGATCCACCCTTTTTT, downstream forward: _UP4_TAATTCGTCTGACAATGAAG

References

Reviews

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