cotE

outer spore coat morphogenetic protein, controls the assembly of the outer spore coat layer

Locus: BSU_17030

Isoelectric point: 4.14

Molecular weight: 20.83 kDa

Protein length: 181 aa

Gene length: 546 bp

Function: assembly of the outer spore coat

Product: spore coat morphogenetic protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class II

Gene

Coordinates: 1,775,067 1,775,612

Phenotypes of a mutant

• mis-assembly of the outer spore coat PubMed
• inactivation of cotE reduces sporulation efficiency to 49.1% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• assembly of the CotC-CotU complex PubMed

Protein family

• cotE family (single member, according to UniProt)

Localization

• encircles the forespore with a biased accumulation on the mother cell-proximal face of the forespore PubMed
• outer spore coat, localization depends on SpoIVA PubMed

Expression and Regulation

Operons

• Genes: cotE
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigK: sigma factor, PubMed, in SigK regulon

  Regulatory mechanism

  • GerE: repression, PubMed, in GerE regulon

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

Biological materials

Mutant

• 1S105 ( cotE::cat), PubMed, available at BGSC
• BKE17030 (cotE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG, downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG
• BKK17030 (cotE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCGGCATGCCTCCTTG, downstream forward: _UP4_TAAAAAAGGGACTAGGGGAG

Labs working on this gene/protein

• Arthur Aronson, Purdue University, West Lafayette, USA homepage
• Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

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Original publications

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