csbB

lipoteichoic acid glycosyltransferase, general stress protein, required for protection against paraquate stress

locus: BSU_08600

pI: 9.20

mw: 37.57 kDa

protein length: 329 aa

gene length: 990 bp

function: lipoteichoic acid glycosylation , survival of stress conditions

product: lipoteichoic acid glycosyltransferase

synonyms: yfhN

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.3|Biosynthesis of lipoteichoic acid]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.2|Biosynthesis of lipoteichoic acid]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 930,818 931,807

The protein

Protein family

• [SW|glycosyltransferase 2 family] (according to UniProt)

Paralogous protein(s)

• [protein|EF18470AD2B07755431534B52A8FCE6754FDBA9D|YkoT], [protein|FD76A781162795EAF174B59C0A90D2ED3ACC0AB7|YkcC]

Structure

• [PDB|5EKP] (from Synechocystis sp., 44% identity) [pubmed|26729507]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]-[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]
  description: [Pubmed|22383849,8921856]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|8921856,9636707], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|9636707], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|8921856,15805528,9636707]

Biological materials

Mutant

• MGNA-C319 (csbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2317 NBRP B. subtilis, Japan]
• BKE08600 ([gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAGGCACCTTCTTTTT, downstream forward: _UP4_TGAAAATACCAAGCGCCATT
• BKK08600 ([gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAGGCACCTTCTTTTT, downstream forward: _UP4_TGAAAATACCAAGCGCCATT

References

• 8921856,9636707,23632331,15805528,22582280,27185829,26729507,29343515