sasB [2019-06-27 14:28:25]

(p)ppGpp synthetase

Locus: BSU11600

Isoelectric point: 6.71

Molecular weight: 24.56 kDa

Protein length: 211 aa

Gene length: 636 bp

Function: ppGpp synthesis independent from stringent response

Product: (p)ppGpp synthetase

Essential: no

Synonyms: yjbM,relQ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides

Gene

Coordinates: 1,237,006 1,237,641

Phenotypes of a mutant

• a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
• a relA sasA sasB triple mutant acquires suppressor mutations in guaA, guaB, gmk or inactivation of codY PubMed

The protein

Protein family

• RelA/SpoT family (with ywac and RelA, according to UniProt)

Paralogous protein(s)

• SasA

Domains

• single ppGpp synthetase domain PubMed

Effectors of protein activity

• allosterically activated by pppGpp PubMed

Structure

• 5DEC PubMed

Expression and Regulation

Operons

• Genes: yjbL-sasB-nadF-yjbO
  Description: PubMed

  Regulation

  • expressed during exponential growth

Biological materials

Mutant

• MGNA-B159 (yjbM::erm), available at the NBRP B. subtilis, Japan
• GP2066 (sasA sasB relA::mls), available in Jörg Stülke's lab
• BKE11600 (sasB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
• BKK11600 (sasB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA

References

Reviews

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Original publications

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