sasB [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

(p)ppGpp synthetase

locus: BSU11600

pI: 6.00

mw: 24.00 kDa

function: ppGpp synthesis independent from stringent response

product: (p)ppGpp synthetase

essential: no

synonyms: yjbM, relQ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]

[SW|Categories] containing this gene/protein

• [SW|metabolism of signalling nucleotides]

Gene

Coordinates on the chromosome (coding sequence): 1,237,006 -> 1,237,641

Phenotypes of a mutant

• a ''[protein|search|relA] [protein|search|sasA] [protein|search|sasB]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[protein|search|guaB]'' or inactivation of ''[protein|search|codY]'' [Pubmed|24163341]
• a ''[protein|search|relA] [protein|search|sasA] [protein|search|sasB]'' triple mutant acquires suppressor mutations in ''[protein|search|guaA], [protein|search|guaB], [protein|search|gmk]'' or inactivation of ''[protein|search|codY]'' [Pubmed|24682323,24163341]

The protein

Paralogous protein(s)

• [protein|search|SasA]

[SW|Domains]

• single ppGpp synthetase domain [Pubmed|24163341]

Effectors of protein activity

• allosterically activated by pppGpp [Pubmed|26460002]

Structure

• [PDB|5DEC] [Pubmed|26460002]

[SW|Interactions]

• forms homotetramers [Pubmed|26460002]

Expression and Regulation

Operon

• ''[protein|search|yjbL]-[protein|search|sasB]-[protein|search|nadF]-[protein|search|yjbO]'' [Pubmed|18067544]

Regulation

• expressed during exponential growth

Biological materials

Mutant

• GP2066 (''[SW|sasA] [SW|sasB] [SW|relA]::mls''), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 27149325

Original publications

• 22950019,18670626,19447912,18067544,24163341,24489751,24682323,26460002,27875634