srfAA

surfactin synthetase / competence

Locus: BSU_03480

Isoelectric point: 4.87

Molecular weight: 401.68 kDa

Protein length: 3588 aa

Gene length: 10764 bp

Function: antibiotic synthesis, biocontrol of fungal growth

Product: surfactin synthetase / competence

Essential: no

Synonyms: comL

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Exponential and early post-exponential lifestyles → Swarming
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 376,968 → 387,731

Phenotypes of a mutant

• altered cell death pattern in colonies PubMed
• reduced swarming of srfAA mutant of B. subtilis NCIB3610 PubMed

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Paralogous protein(s)

• partial match (more than 1,000 amino acids): DhbF, PksJ, PksN, PpsA, PpsB, PpsE, SrfAC

Domains

• 3 Carrier domains (aa 971-1046, aa 2010-2085, aa 3038-3112) (according to UniProt)

Modification

• phosphorylation on Ser-1006 PubMed

Structure

• 2VSQ (termination module of SrfAC, 35% identity) PubMed

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: srfAA-srfAB-comS-srfAC-srfAD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PhoP: activation, PubMed, in PhoP regulon
  • CodY: repression, PubMed, in CodY regulon
  • ComA: activation, PubMed, in ComA regulon
  • PerR: activation, PubMed, in PerR regulon
  • Spx: repression, PubMed, in Spx regulon
  • Abh: repression, PubMed, in Abh regulon

  Regulation

  • expressed at high cell density (ComA) PubMed

  Additional information

  • A ncRNA is predicted between 'hxlR' and 'srfAA' PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• BKE03480 (ΔsrfAA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGTCATACCTCCCCT, downstream forward: _UP4_GAAATTTTCTAATAGAGGTG
• BKK03480 (ΔsrfAA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGTCATACCTCCCCT, downstream forward: _UP4_GAAATTTTCTAATAGAGGTG

References

Reviews

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Original publications

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