ywaA [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

branched-chain amino acid aminotransferase

Locus: BSU38550

Isoelectric point: 4.00

Molecular weight: 40.00 kDa

Function: biosynthesis of branched-chain amino acids

Product: branched-chain amino acid aminotransferase

Essential: no

E.C.: 2.6.1.42

Synonyms: ipa-0r

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids

Categories containing this gene/protein

• biosynthesis/ acquisition of amino acids, most abundant proteins

This gene is a member of the following regulons

• CodY regulon

Gene

Coordinates on the chromosome (coding sequence): 3,957,391 -> 3,958,482

The protein

Catalyzed reaction/ biological activity

• L-leucine 2-oxoglutarate = 4-methyl-2-oxopentanoate L-glutamate (according to Swiss-Prot)

Protein family

• class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

Paralogous protein(s)

• YbgE

Modification

• S-cysteinylation after diamide stress (C104) PubMed

Structure

• 3HT5 (from Mycobacterium tuberculosis, 42% identity, 58% similarity) PubMed

Expression and Regulation

Operon

• ywaA PubMed

Regulation

• repressed during growth in the presence of branched chain amino acids (CodY) PubMed

Regulatory mechanism

• CodY: transcription repression PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Biological materials

Mutant

• a ywaA::spc mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab

References

Loading