ywaA

branched-chain amino acid aminotransferase

locus: BSU_38550

pI: 4.95

mw: 40.16 kDa

protein length: 363 aa

gene length: 1092 bp

function: biosynthesis of branched-chain amino acids

product: branched-chain amino acid aminotransferase

essential: no

ec: 2.6.1.42

synonyms: ipa-0r

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]

Gene

Coordinates: 3,957,391 3,958,482

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
• 2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
• 2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)

Protein family

• [SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

Paralogous protein(s)

• [protein|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|YbgE]

Modification

• S-cysteinylation after diamide stress (C104) [Pubmed|17611193]

[SW|Cofactors]

• PLP

Structure

• [PDB|3HT5] (from '' Mycobacterium tuberculosis'', 42% identity, 58% similarity) [Pubmed|19923721]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|8163996FCB9D02F771E7A04A91D5720027260F12|ywaA]
  description: [Pubmed|9353933]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24163341], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|24163341]

Biological materials

Mutant

• MGNA-B757 (ywaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1756 NBRP B. subtilis, Japan]
• a ''ywaA::spc'' mutant and a ''[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd] [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE] [gene|8163996FCB9D02F771E7A04A91D5720027260F12|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
• BKE38550 ([gene|8163996FCB9D02F771E7A04A91D5720027260F12|ywaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAATCTCCCTGCTG, downstream forward: _UP4_TAAGAAAAAAGCCGGCCCAT
• BKK38550 ([gene|8163996FCB9D02F771E7A04A91D5720027260F12|ywaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAATCTCCCTGCTG, downstream forward: _UP4_TAAGAAAAAAGCCGGCCCAT

References

• 12670965,17611193,24163341,15378759,28516784