ribT

similar to dioxin reductive etherase

locus: BSU_23240

pI: 5.75

mw: 14.43 kDa

protein length: 124 aa

gene length: 375 bp

function: unknown

product: dioxin reductive etherase

essential: no

synonyms: ribD

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,427,405 2,427,779

The protein

[SW|Domains]

• [SW|N-acetyltransferase domain] (aa 3-124) (according to UniProt)

Structure

• [PDB|5XXS] [pubmed|29241954]

Expression and Regulation

Operons

• genes: [gene|3DFCF7C5FE15C9A3E9E7E4B0DA8E926B85DBD180|ribD]-[gene|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE]-[gene|F07B7062C850106A95C15978E36DA500F8B089D6|ribA]-[gene|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH]-[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]
  description: [Pubmed|8159171]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8159171], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|FMN-box|FMN-box]: transcription termination, via [SW|FMN-box] in the presence of FMN or FMNH₂, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|RibR], in [regulon|FMN-box|FMN-box]

  regulation

  • expressed in the absence of FMN ([SW|FMN-box]) [Pubmed|15808508]
  • binding of FMN to the [SW|FMN-box] RNA is facilitated by [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|NusG]-mediated transcription pausing [pubmed|32817529]
  • the [SW|FMN-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• BKE23240 ([gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACCCCTCTATATC, downstream forward: _UP4_TAAGCAGAGGCTGTGATCAG
• BKK23240 ([gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACCCCTCTATATC, downstream forward: _UP4_TAAGCAGAGGCTGTGATCAG

References

• 12456892,15808508,23270261,8159171,7934830,24442413,25349719,26494285,29241954,29805114