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cmoJ [2018-09-12 14:19:07]
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cmoJ [2018-09-12 14:19:07]

alkyl monooxygenase, required for the conversion of S-methyl-cysteine to cysteine
locus
BSU29310
pI
5.72
mw
49.25 kDa
protein length
442 aa Sequence Blast
gene length
1326 bp Sequence Blast
function
utilization of S-methyl-cysteine
product
alkyl monooxygenase
essential
no
synonyms
ytnJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.1|Conversion of S-methyl cysteine to cysteine]

    • Gene

    Coordinates
    3,001,724 → 3,003,052

    Phenotypes of a mutant

  • no growth with S-methyl cysteine [Pubmed|23944997]

    • The protein

    Catalyzed reaction/ biological activity

  • oxidation of S-methyl-cysteine [Pubmed|23944997]

    • Protein family

  • ntaA/snaA/soxA(dszA) monooxygenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|YxeK]

    • [SW|Cofactors]

  • FMN

    • Structure

  • [PDB|1YW1] (complex with FMN), [PDB|1TVL], [PDB|6ASK]

    • Expression and Regulation

    Operons

    genes
    [gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
    description
    [Pubmed|15668000]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15272571], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16109943], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|741156D495BE3857683C8A0390764EAD83845ABC|AscR]: activation, [Pubmed|16109943], in [regulon|741156D495BE3857683C8A0390764EAD83845ABC|AscR regulon]

    • regulation

  • induced in the presence of methionine and taurine [Pubmed|11390694]
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    Biological materials

    Mutant

  • MGNA-A155 (ytnJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/155 NBRP B. subtilis, Japan]
  • BKE29310 (Δ[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAACTGAATAAAATCTGCAC, downstream forward: _UP4_TAGGTTTCGTCTGATCGAAT
  • BKK29310 (Δ[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAACTGAATAAAATCTGCAC, downstream forward: _UP4_TAGGTTTCGTCTGATCGAAT

    • References

  • 16109943,15668000,15272571,23944997