succinate dehydrogenase (cytochrome b558 subunit)
product
succinate dehydrogenase (cytochrome b558 subunit)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|carbon core metabolism], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|FsrA regulon]Gene
Coordinates on the chromosome (coding sequence)
2,908,129 -> 2,908,737
Phenotypes of a mutant
defective in the the ability to support the growth of ''Synechococcus leopoliensis'' CCAP1405/1 on agar media [Pubmed|25875741]The protein
Protein family
cytochrome b558 family (according to Swiss-Prot)[SW|Cofactors]
FeEffectors of protein activity
Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [Pubmed|3910107]Activated by Cytochrome b558 [Pubmed|6799760]Structure
[PDB|1NEK] (''E. coli'')[SW|Localization]
membrane protein [Pubmed|18763711][SW|Interactions]
[protein|search|SdhB]-[protein|search|SdhC] (as part of the [protein|search|SdhC]-[protein|search|SdhB]-[protein|search|SdhA] complex)part of a [SW|respiratory supercomplex] [Pubmed|23880299]Additional information
This enzyme is a trimer membrane-bound [Pubmed|3910107] [Pubmed|6799760]One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane [Pubmed|3910107] [Pubmed|6799760]Another subunit is the flavoprotein one, required for FAD usage [Pubmed|3910107] [Pubmed|6799760]The other subunit has an iron-sulphur domain necessary for the catalytic activity [Pubmed|3910107] [Pubmed|6799760]extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/Succinate_Dehydrogenase Proteopedia]Expression and Regulation
Operon
''[protein|search|sdhC]''-''[protein|search|sdhA]''-''[protein|search|sdhB]'' [Pubmed|3036777][SW|Sigma factor]
[protein|search|SigA] [Pubmed|2495271]Regulation
SdhC is less expressed under conditions of extreme iron starvation ([protein|search|FsrA]) [Pubmed|18697947]part of the iron sparing response ([protein|search|FsrA]) [Pubmed|22389480]Regulatory mechanism
[protein|search|FsrA]: translation inhibition [Pubmed|22389480]Biological materials
Mutant
GP743 (''[protein|search|sdhC]-[protein|search|sdhA]'', cat), available in [SW|Jörg Stülke]'s labGP792 (''[protein|search|sdhC]-[protein|search|sdhA]-[protein|search|sdhB]''::''phleo''), available in [SW|Jörg Stülke]'s labGP2342 (''[protein|search|sdhC]-[protein|search|sdhA]-[protein|search|sdhB]''::''kan''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s labGP2343 (''[protein|search|sdhC]-[protein|search|sdhA]-[protein|search|sdhB]''::''lox72''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s labtwo-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s labReferences
Reviews
Original publications
6401289,1707123,2120540,7669765,18697947,1324713,3036777,2495271,2176107,3117551,10913269,18763711,12560550,18697947,3910107,6799760,21641999,22389480,23880299,25875741 
