sdhC

succinate dehydrogenase (cytochrome b558 subunit)

locus: BSU_28450

pI: 9.83

mw: 22.79 kDa

protein length: 202 aa

gene length: 609 bp

function: TCA cycle

product: succinate dehydrogenase (cytochrome b558 subunit)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,908,129 2,908,737

Phenotypes of a mutant

• defective in the the ability to support the growth of ''Synechococcus leopoliensis'' CCAP1405/1 on agar media [Pubmed|25875741]
• defective in biofilm formation [pubmed|31420537]

The protein

Protein family

• cytochrome b558 family (single member, according to UniProt)

[SW|Cofactors]

• Fe

Effectors of protein activity

• Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [Pubmed|3910107]
• Activated by Cytochrome b558 [Pubmed|6799760]

Structure

• [PDB|1NEK] (''E. coli'') [pubmed|12560550]

[SW|Localization]

• membrane protein [Pubmed|18763711]

Additional information

• This enzyme is a trimer membrane-bound [Pubmed|3910107] [Pubmed|6799760]
• One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane [Pubmed|3910107] [Pubmed|6799760]
• Another subunit is the flavoprotein one, required for FAD usage [Pubmed|3910107] [Pubmed|6799760]
• The other subunit has an iron-sulphur domain necessary for the catalytic activity [Pubmed|3910107] [Pubmed|6799760]
• extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/Succinate_Dehydrogenase Proteopedia]

Expression and Regulation

Operons

• genes: [gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]
  description: [Pubmed|3036777]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2495271], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • constitutive

Other regulations

• [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]: translation inhibition, [Pubmed|22389480]

additional information

• highly expressed at high iron concentrations [pubmed|31420537]

Biological materials

Mutant

• MGNA-B028 (sdhC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1027 NBRP B. subtilis, Japan]
• GP743 ∆(''[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]'')::cat, available in [SW|Jörg Stülke]'s lab
• GP792 ∆(''[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]'')::''phleo'', available in [SW|Jörg Stülke]'s lab
• GP2342 ∆(''[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]'')::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
• GP2343 ∆(''[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]'')::''lox72'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
• BKE28450 (∆[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTACCCCCTGTTT, downstream forward: _UP4_TAAGAGTACTAGATTACTAG
• BKK28450 (∆[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTACCCCCTGTTT, downstream forward: _UP4_TAAGAGTACTAGATTACTAG

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 11803018

Original publications

• 6401289,1707123,2120540,7669765,18697947,1324713,3036777,2495271,2176107,3117551,10913269,18763711,12560550,18697947,3910107,6799760,21641999,22389480,23880299,25875741,12560550,31420537