N-acetylglucosamine-malate deacetylase, minor enzyme involved in bacillithiol synthesis, may act as amidase for the processing of BSH conjugates
function
biosynthesis of bacillithiol
product
N-acetylglucosamin-malate deacetylase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress][SW|Categories] containing this gene/protein
[SW|miscellaneous metabolic pathways], [SW|resistance against oxidative and electrophile stress]This gene is a member of the following [SW|regulons]
[SW|Spx regulon]Gene
Coordinates on the chromosome (coding sequence)
2,121,641 -> 2,122,306
The protein
Paralogous protein(s)
[SW|BshB1]Expression and Regulation
Operon
''[protein|search|yojE]-[protein|search|yoyC]-[protein|search|yojF]-[SW|bshB2]'' (minor transcript) [Pubmed|23894131]''[protein|search|yoyC]-[protein|search|yojF]-[SW|bshB2]'' (major transcript) [Pubmed|23894131,20308541][SW|Sigma factor]
''[protein|search|yojE]'': [protein|search|SigA] [Pubmed|23894131]''[protein|search|yoyC]'': [protein|search|SigA] [Pubmed|23894131]Regulation
''[protein|search|yoyC]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]Regulatory mechanism
[protein|search|Spx]: transcription activation [Pubmed|23894131]Biological materials
Mutant
''bshB2'' null mutant and ''[SW|bshB1]'' / ''[SW|bshB2]'' double null mutant available in [SW|John Helmann] labCS212 (''[protein|search|bshB2]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]References
20308541,19578333,23894131,23758290,27197833 
