folC

folyl-polyglutamate synthetase

locus: BSU_28080

pI: 5.81

mw: 48.00 kDa

protein length: 430 aa

gene length: 1293 bp

function: biosynthesis of folate

product: folyl-polyglutamate synthetase

essential: yes

ec: 6.3.2.17

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,865,312 2,866,604

Phenotypes of a mutant

• essential [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• 7,8-dihydropteroate + ATP + L-glutamate --> 7,8-dihydrofolate + ADP + H⁺ + phosphate (according to UniProt)
• (6S)-5,6,7,8-tetrahydrofolyl-(γ-L-Glu)_n + ATP + L-glutamate --> (6S)-5,6,7,8-tetrahydrofolyl-(γ-L-Glu)_n+1 + ADP + H⁺ + phosphate (according to UniProt)

Protein family

• Folylpolyglutamate synthase family (single member, according to UniProt)

Structure

• [PDB|1FGS] (from ''Lactobacillus casei'', 32% identity, 51% similarity) [Pubmed|9618466]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|3E84B3AC08D0B314286CEF38534BD81B568F3E0E|valS]-[gene|80502F76F076C08CCE702F25095C06D14CD8154A|folC]
  description: [Pubmed|9098041]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9098041], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: transcription termination/antitermination, via [SW|riboswitch], in [regulon|T-box|T-box]

  regulation

  • induced by valine limitation ([SW|T-box]) [Pubmed|9098041,19258532]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

References

Reviews

• 19258532

Original Publications

• 9098041,28189581