fbaA [2019-02-19 19:42:33]

fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme

locus: BSU37120

pI: 5.03

mw: 30.25 kDa

protein length: 285 aa

gene length: 858 bp

function: enzyme in glycolysis/ gluconeogenesis

product: fructose-1,6-bisphosphate aldolase

essential: no

ec: 4.1.2.13

synonyms: fba,fba1,tsr

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

Gene

Coordinates: 3,808,512 3,809,369

Phenotypes of a mutant

• essential [Pubmed|12682299], non-essential according to [Pubmed|23420519]

The protein

Catalyzed reaction/ biological activity

• D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)

Protein family

• class II fructose-bisphosphate aldolase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|IolJ]

[SW|Domains]

• 2 x Dihydroxyacetone phosphate binding domain (210212), (231234)

Modification

• phosphorylation on Thr-212 and Thr-234 [Pubmed|17218307]

[SW|Cofactors]

• Zn2+ (Metalloenzyme)

Effectors of protein activity

• Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate [Pubmed|24624] [Pubmed|15125960]
• Activated by NH4+ [Pubmed|15125960]

Structure

• [PDB|3Q94] (from ''Bacillus anthracis'')

Additional information

• Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
• extensive information on the structure and enzymatic properties of FbaA can be found at [http://www.proteopedia.org/wiki/index.php/Fructose_Bisphosphate_Aldolase Proteopedia]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]
  description: [Pubmed|11489127]

  regulation

  • constitutively expressed [Pubmed|11489127]

• genes: [gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]
  description: [Pubmed|11489127]

  regulation

  • constitutively expressed [Pubmed|11489127]

Biological materials

Mutant

• GP591 (''fbaA''::''cat''), available in [SW|Jrg Stlke]'s lab, [Pubmed|23420519]
• GP596 (''fbaA''::''erm''), available in [SW|Jrg Stlke]'s lab, [Pubmed|23420519]
• BKE37120 ([gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
• BKK37120 ([gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT

Expression vectors

• for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1423, available in [SW|Jrg Stlke]'s lab
• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP88, available in [SW|Jrg Stlke]'s lab, [pubmed|19193632]
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP395, available in [SW|Jrg Stlke]'s lab

lacZ fusion

• pGP601 (in [protein|search|pAC6]) [pubmed|11489127]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab, [pubmed|19193632]

References

Reviews

• 25267444

Original publications

• 17218307,15125960,24624,16843441,11489127,20525796,23420519,23033921,24571712,15378759