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fbaA [Mon May 16 2016 15:48:26 GMT+0200 (CEST)]
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fbaA [Mon May 16 2016 15:48:26 GMT+0200 (CEST)]

fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme
locus
BSU37120
pI
5.00
mw
30.00 kDa
function
enzyme in glycolysis/ gluconeogenesis
product
fructose-1,6-bisphosphate aldolase
essential
no
ec
4.1.2.13
synonyms
fba, fba1, tsr
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,808,512 -> 3,809,369

    Phenotypes of a mutant

  • essential [Pubmed|12682299], non-essential according to [Pubmed|23420519]

    • The protein

    Catalyzed reaction/ biological activity

  • D-fructose 1,6-bisphosphate = glycerone phosphate D-glyceraldehyde 3-phosphate (according to Swiss-Prot)

    • Protein family

  • class II fructose-bisphosphate aldolase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|FbaB]

    • [SW|Domains]

  • 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)

    • Modification

  • phosphorylation on Thr-212 and Thr-234 [Pubmed|17218307]

    • [SW|Cofactors]

  • Zn2 (Metalloenzyme)

    • Effectors of protein activity

  • Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate [Pubmed|24624] [Pubmed|15125960]
  • Activated by NH4 [Pubmed|15125960]

    • Structure

  • [PDB|3Q94] (from ''Bacillus anthracis'')

    • Additional information

  • Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
  • extensive information on the structure and enzymatic properties of FbaA can be found at [http://www.proteopedia.org/wiki/index.php/Fructose_Bisphosphate_Aldolase Proteopedia]

    • Expression and Regulation

    Operon

  • ''fbaA'' [Pubmed|11489127]
  • ''[protein|search|fbaA]-[protein|search|ywjH]'' [Pubmed|11489127]

    • Regulation

  • constitutively expressed [Pubmed|11489127]
  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]

    • Biological materials

    GP591 (''fbaA''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]

  • GP596 (''fbaA''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]

    • Expression vector

  • for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1423, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP88, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP395, available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP601 (in [protein|search|pAC6])

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

  • 17218307,15125960,24624,16843441,11489127,20525796,23420519,23033921,24571712,15378759