ykuK [2018-10-11 18:34:34]

unknown

locus: BSU14110

pI: 6.42

mw: 19.34 kDa

protein length: 172 aa

gene length: 516 bp

function: unknown

product: unknown, distantly related to RNase H

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,484,466 → 1,484,984

The protein

[SW|Domains]

• [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?ascbin=8&maxaln=10&seltype=2&uid=pfam04308 DUF458]

Structure

• [PDB|2E3U] (from Pyrococcus horikoshii, 24% identity)

Expression and Regulation

Operons

• genes: [gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
  description: [Pubmed|16395550]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]~P [Pubmed|18840696,14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16395550,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• MGNA-A772 (ykuK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/772 NBRP B. subtilis, Japan]
• GP214 (deletion of ''[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]'', replaced by ''aphA3''), available in the [SW|Stülke] lab
• BKE14110 (Δ[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGACACTCCTTTTCA, downstream forward: _UP4_TAATTTGGCTGAAAAAGCCA
• BKK14110 (Δ[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGACACTCCTTTTCA, downstream forward: _UP4_TAATTTGGCTGAAAAAGCCA

lacZ fusion

• pGP709 (in [protein|search|pAC5]), available in [SW|Stülke] lab, pGP713 (in [protein|search|pAC6]), available in [SW|Stülke] lab

References

• 16395550,16165328