pnbA

para-nitrobenzyl esterase

Locus: BSU_34390

Isoelectric point: 4.78

Molecular weight: 53.82 kDa

Protein length: 489 aa

Gene length: 1470 bp

Function: lipid degradation

Product: para-nitrobenzyl esterase

Essential: no

E.C.: 3.1.1.3

Synonyms: estB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of lipids/ other
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 3,530,635 3,532,104

The protein

Protein family

• type-B carboxylesterase/lipase family (single member, according to UniProt)

Modification

• phosphorylation on Ser-189 PubMed

Structure

• 1C7J PubMed

Expression and Regulation

Operons

• Genes: pnbA
  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • repressed during logarithmic growth (AbrB) PubMed

• Genes: slrR-pnbA
  Description: PubMed

  Regulatory mechanism

  • Abh: activation, PubMed, in Abh regulon
  • SinR: repression, PubMed, in SinR regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Additional information

  • induction by sequestration of SinR by SinI, SlrA PubMed or by SlrR itself PubMed

Biological materials

Mutant

• GP951 (pnbA::cat), available in Jörg Stülke's lab
• GP955 (slrR-pnbA::cat), available in Jörg Stülke's lab
• BKE34390 (pnbA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT, downstream forward: _UP4_TAAATATGGGGAAAACAGGA
• BKK34390 (pnbA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT, downstream forward: _UP4_TAAATATGGGGAAAACAGGA

References

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