argD

acetylornithine transaminase

Locus: BSU_11220

Isoelectric point: 5.93

Molecular weight: 40.74 kDa

Protein length: 385 aa

Gene length: 1155 bp

Function: biosynthesis of arginine

Product: acetylornithine transaminase

Essential: no

E.C.: 2.6.1.11

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of arginine

Gene

Coordinates: 1,198,099 → 1,199,256

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + N²-acetyl-L-ornithine --> L-glutamate + N-acetyl-L-glutamate 5-semialdehyde (according to UniProt)

Protein family

• Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 2EH6 (from Aquifex aeolicus, 45% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: argC-argJ-argB-argD-carA-carB-argF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AhrC: repression, PubMed, in AhrC regulon
  • CodY: repression, PubMed, in CodY regulon
  • YlxR: regulation, PubMed, in YlxR regulon

  Regulation

  • repressed by arginine (AhrC) PubMed
  • induced in the absence of YlxR PubMed

Biological materials

Mutant

• BKE11220 (ΔargD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTCATGAAACAGCCTCCTT, downstream forward: _UP4_TGATTTTTTTTCGATATAAA
• BKK11220 (ΔargD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTCATGAAACAGCCTCCTT, downstream forward: _UP4_TGATTTTTTTTCGATATAAA

References

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