walR [2019-08-28 12:30:41]

two-component response regulator ([SW|OmpR family]), controls cell wall metabolism

locus: BSU_40410

pI: 4.88

mw: 27.08 kDa

protein length: 235 aa

gene length: 708 bp

function: control of cell wall metabolism

product: two-component response regulator ([SW|OmpR family])

essential: yes

synonyms: yycF

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 4,153,696 4,154,403

Phenotypes of a mutant

• essential [Pubmed|12682299]
• essentiality can be bypassed by constitutive expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] or [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] in a [gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC] [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] mutant background [pubmed|29465029]
• a constitutively active form of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] ([protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]-R204C) results in constitutive expression of the [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] regulon, and in combination with a ''[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]'' mutation to the formation of cell wall-less L-forms [Pubmed|22122227]

The protein

Protein family

• [SW|OmpR family] of two-component response regulators

Paralogous protein(s)

• [protein|6A37531896205A9B894C81AB0563C216C0B52CD7|YkoG], [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|YclJ], [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]

Modification

• phosphorylated by [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK] on an Asp residue
• phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] on Thr-101, this stimulates [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] activity [Pubmed|26102633]

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• [PDB|2OQR] (from Mycobacterium tuberculosis, 48% identity) [pubmed|17942407]
• [PDB|2D1V] (DNA binding domain)[PDB|2ZWM] (receiver domain)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000217 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
  description: [Pubmed|9829949]

  regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]

Biological materials

Mutant

• MGNA-B828 (yycF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1827 NBRP B. subtilis, Japan]

Expression vectors

• pGP2633, constitutive expression in ''B. subtilis'' (based on [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
• pGP2635, expression of His-[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] in ''E. coli'' (based on [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Kevin Devine], Dublin

References

Reviews

• 19019149,18245295,18792692,22126136,10094672,28886686

The [SW|WalR regulon]

• 17581128,10878122,12950927,23199363

Other original Publications

• 18573169,17350627,10094672,17827301,9829949,16030236,20111065,20167622,20834167,24125693,22122227,24125693,24490131,18789936,26102633,17942407,29465029,31339578