walR

two-component response regulator (OmpR family), controls cell wall metabolism

Locus: BSU_40410

Isoelectric point: 4.88

Molecular weight: 27.08 kDa

Protein length: 235 aa

Gene length: 708 bp

Function: control of cell wall metabolism

Product: two-component response regulator (OmpR family)

Essential: yes

Synonyms: yycF

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall/ other
• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 4,153,696 4,154,403

Phenotypes of a mutant

• essential PubMed
• essentiality can be bypassed by constitutive expression of lytE or cwlO in a pdaC iseA mutant background PubMed
• a constitutively active form of WalR (WalR-R204C) results in constitutive expression of the WalR regulon, and in combination with a sepF mutation to the formation of cell wall-less L-forms PubMed

The protein

Protein family

• OmpR family of two-component response regulators

Paralogous protein(s)

• YkoG, YclJ, PhoP

Domains

• Response regulatory domain (aa 4-117) (according to UniProt)

Modification

• phosphorylated by WalK on Asp-53
• phosphorylated by PrkC on Thr-101, this stimulates WalR activity PubMed

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• 2OQR (from Mycobacterium tuberculosis, 48% identity) PubMed
• 2D1V (DNA binding domain)2ZWM (receiver domain)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: walR-walK-walH-walI-walJ-htrC
  Description: PubMed

  Regulation

  • htrC transcript expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-B828 (yycF::erm), available at the NBRP B. subtilis, Japan

Expression vectors

• pGP2633, constitutive expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab
• pGP2635, expression of His-WalR in E. coli (based on pWH844), available in Jörg Stülke's lab

Labs working on this gene/protein

• Kevin Devine, Dublin

References

Reviews

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The WalR regulon

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Other original Publications

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