citS [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

two-component sensor kinase, regulation of citrate uptake

locus: BSU07580

pI: 6.00

mw: 59.00 kDa

function: regulation of citrate uptake

product: two-component sensor kinase

essential: no

synonyms: yflR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources], [SW|protein modification], [SW|transcription factors and their control], [SW|membrane proteins], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence): 830,945 -> 832,573

Phenotypes of a mutant

• no growth with citrate as sole carbon source [Pubmed|10972810]

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|search|CitT]

[SW|Domains]

• two transmembrane segments
• [SW|PAS domain]
• C-terminal histidine phosphotransferase domain

Modification

• autophosphorylation on a His residue

[SW|Localization]

• cell membrane (Heterogeneous) [Pubmed|16479537]

[SW|Interactions]

• [protein|search|CitS]-[protein|search|CitT]

Expression and Regulation

Operon

• ''[protein|search|citS]-[protein|search|citT]-[protein|search|yflP]'' [Pubmed|10972810]

Biological materials

Mutant

• MGNA-C333 (citS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2331 NBRP B. subtilis, Japan]

References

• 10094672,16842348,10972810,16479537