citS

two-component sensor kinase, regulation of citrate uptake

Locus: BSU_07580

Isoelectric point: 6.48

Molecular weight: 59.72 kDa

Protein length: 542 aa

Gene length: 1629 bp

Function: regulation of citrate uptake

Product: two-component sensor kinase

Essential: no

Synonyms: yflR

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Two-component sensor kinase
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Gene

Coordinates: 830,945 832,573

Phenotypes of a mutant

• no growth with citrate as sole carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of CitT
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• two transmembrane segments
• PAS domain
• Histidine kinase domain (aa 336-528) (according to UniProt)

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• activity may be controlled by interaction with YflP PubMed

Localization

• cell membrane (Heterogeneous) PubMed

Expression and Regulation

Operons

• Genes: citS-citT-yflP
  Description: PubMed

Biological materials

Mutant

• MGNA-C333 (citS::erm), available at the NBRP B. subtilis, Japan
• BKE07580 (citS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT, downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA
• BKK07580 (citS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT, downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA

References

Reviews

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Original publications

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