gntZ

6-phosphogluconate dehydrogenase

Locus: BSU_40080

Isoelectric point: 4.76

Molecular weight: 51.82 kDa

Protein length: 468 aa

Gene length: 1407 bp

Function: gluconate utilization

Product: 6-phosphogluconate dehydrogenase

Essential: no

E.C.: 1.1.1.44

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of gluconate

Gene

Coordinates: 4,117,080 4,118,486

The protein

Catalyzed reaction/ biological activity

• 6-phospho-D-gluconate + NAD⁺ --> CO₂ + D-ribulose 5-phosphate + NADH (according to UniProt)

Protein family

• 6-phosphogluconate dehydrogenase family (with YqeC and GndA, according to UniProt)

Paralogous protein(s)

• YqeC, GndA

Structure

• 2W8Z (from Geobacillus stearothermophilus, 58% identity) PubMed

Expression and Regulation

Operons

• Genes: gntR-gntK-gntP-gntZ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GntR: repression, PubMed, in GntR regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • AbrB: activation, PubMed, in AbrB regulon

  Regulation

  • induced by gluconate (GntR) PubMed

• Genes: gntZ
  Description: PubMed

  Regulation

  • induced by gluconate (GntR) PubMed

Biological materials

Mutant

• MGNA-B679 (gntZ::erm), available at the NBRP B. subtilis, Japan
• BKE40080 (gntZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTTACAGCTCCTTCT, downstream forward: _UP4_TAACCTGTATTAAAAACACG
• BKK40080 (gntZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTTACAGCTCCTTCT, downstream forward: _UP4_TAACCTGTATTAAAAACACG

References

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