carB

carbamoyl-phosphate transferase-arginine (subunit B)

locus: BSU_11240

pI: 4.71

mw: 112.75 kDa

protein length: 1030 aa

gene length: 3093 bp

function: biosynthesis of arginine

product: carbamoyl-phosphate transferase-arginine (subunit B)

essential: no

ec: 6.3.5.5

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]

Gene

Coordinates: 1,200,381 1,203,473

The protein

Catalyzed reaction/ biological activity

• 2 ATP + H₂O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• carB family (with [protein|D57DC8A53E52BC4A2EA894E3C9FD983592882918|PyrAB], according to UniProt)

Paralogous protein(s)

• [protein|D57DC8A53E52BC4A2EA894E3C9FD983592882918|PyrAB]

[SW|Domains]

• 2 [SW|ATP-grasp doamin]s (aa 133-327, aa 675-863) (according to UniProt)
• MGS-like domain (aa 925-1027) (according to UniProt)

Structure

• [PDB|1JDB] (from ''Escherichia coli'', 47% identity, 64% similarity) [Pubmed|10089390]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]-[gene|0FF858E44B3C557EE2C8B3749A458F152E1C535C|argJ]-[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]-[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]-[gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]-[gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]-[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]
  description: [Pubmed|7511775]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7511775], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC]: repression, [Pubmed|1312212], in [regulon|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24843172], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]

  regulation

  • repressed by arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC]) [Pubmed|1312212]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

Biological materials

Mutant

• BKE11240 ([gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATGCTTGAAATACTGG, downstream forward: _UP4_CTCTATAAAAAGGAAGTGGC
• BKK11240 ([gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATGCTTGAAATACTGG, downstream forward: _UP4_CTCTATAAAAAGGAAGTGGC

References

• 6096675,12107147,15378759,24843172,1312212,7511775