mtrA

methylthioribose transporter

Locus: BSU_07340

Isoelectric point: 9.82

Molecular weight: 48.74 kDa

Protein length: 461 aa

Gene length: 1386 bp

Function: uptake of methylthioribose

Product: methylthioribose transporter

Essential: no

Synonyms: yfnA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Other transporters
• Metabolism → Additional metabolic pathways → Sulfur metabolism → sulfur metabolism/ general
• Groups of genes → Membrane proteins

Gene

Coordinates: 805,456 806,841

The protein

Catalyzed reaction/ biological activity

• uptake of methylthioribose PubMed

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

Paralogous protein(s)

• BcaP

Structure

• 5OQT (from Geobacillus kaustophilus, 60% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: mtrA-yfnB
  Description: PubMed

• Genes: mtrA
  Description: PubMed

Biological materials

Mutant

• GP2379 ∆mtrA::kan, available in Jörg Stülke's lab PubMed
• MGNA-C231 (yfnA::erm), available at the NBRP B. subtilis, Japan
• BKE07340 (mtrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAAAAGTTCCTCCTAGA, downstream forward: _UP4_TAATCTCTTTTCAGCCGGCG
• BKK07340 (mtrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAAAAGTTCCTCCTAGA, downstream forward: _UP4_TAATCTCTTTTCAGCCGGCG

Expression vector

• pGP2281: expression of mtrA by pBQ200 in B. subtilis, available in Jörg Stülke's lab

LacZ fusion

• pGP2278 (in pAC5) (GP2964), available in Jörg Stülke's lab

References

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