iolW

scyllo-inositol dehydrogenase, general stress protein

Locus: BSU_33530

Isoelectric point: 5.46

Molecular weight: 39.95 kDa

Protein length: 358 aa

Gene length: 1077 bp

Function: utilization of scyllo-inosose

Product: scyllo-inositol dehydrogenase

Essential: no

Synonyms: yvaA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of inositol
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)

Gene

Coordinates: 3,444,329 3,445,405

The protein

Catalyzed reaction/ biological activity

• NADPH-dependent conversion of scyllo-inosose to scyllo-inositol PubMed
• NADP⁺ + scyllo-inositol --> H⁺ + NADPH + scyllo-inosose (according to UniProt)

Protein family

• Gfo/Idh/MocA family (according to UniProt)

Paralogous protein(s)

• IolU, IolG, YrbE, IolX, YfiI, NtdC, YteT

Cofactors

• NADP⁺ PubMed

Structure

• 3GFG

Expression and Regulation

Operons

• Genes: iolW
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-A443 (iolU::erm), available at the NBRP B. subtilis, Japan
• BKE33530 (iolW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAGAAGTATATACCCTC, downstream forward: _UP4_TAAAACAAAAGCCTCCCCAA
• BKK33530 (iolW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAGAAGTATATACCCTC, downstream forward: _UP4_TAAAACAAAAGCCTCCCCAA

References

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