SubtiBank SubtiBank
yxeQ
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yxeQ

cis-aconitate decarboxylase
Locus
BSU_39460
Isoelectric point
5.94
Molecular weight
47.64 kDa
Protein length
445 aa Sequence Blast
Gene length
1338 bp Sequence Blast
Function
metabolite conversion
Product
cis-aconitate decarboxylase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
4,055,536 4,056,873

The protein

Catalyzed reaction/ biological activity

  • cis-aconitate --> itaconate + CO2 PubMed
  • Protein family

  • prpD family (with MmgE, according to UniProt)
  • Structure

    Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • Regulation

  • repressed in the presence of cysteine (CymR) PubMed
  • Additional information

  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B713 (yxeQ::erm), available at the NBRP B. subtilis, Japan
  • BKE39460 (yxeQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCTGCTGTCAGCCCTTGTT, downstream forward: _UP4_TAAGATCAACTCTATTCGGT
  • BKK39460 (yxeQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCTGCTGTCAGCCCTTGTT, downstream forward: _UP4_TAAGATCAACTCTATTCGGT
  • References

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