ywbH

holin-like protein

Locus: BSU_38320

Isoelectric point: 9.59

Molecular weight: 14.12 kDa

Protein length: 128 aa

Gene length: 387 bp

Function: unknown

Product: holin-like protein

Essential: no

Synonyms: ipa-23r,cidA

Genomic Context

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Categories containing this gene/protein

• Groups of genes → Membrane proteins
• Groups of genes → Proteins of unknown function

Gene

Coordinates: 3,933,209 3,933,595

Phenotypes of a mutant

• a ywbH-ywbG mutant is delayed in pellicle formation PubMed
• a ywbH-ywbG yxaK-yxaC pftA-pftB triple mutant is severely delayed in pellicle formation PubMed
• a ywbH-ywbG overexpression strain has more robust pellicle formation PubMed

The protein

Protein family

• CidA/LrgA family (with PftA, according to UniProt)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ywbH-ywbG
  Description: PubMed

  Regulatory mechanism

  • YwbI: activation, PubMed, in YwbI regulon

  Regulation

  • induced by acetate (YwbI) PubMed

Biological materials

Mutant

• MGNA-B225 (ywbH::erm), available at the NBRP B. subtilis, Japan
• BKE38320 (ywbH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGATGCCTCCCTTAT, downstream forward: _UP4_AAGAGAAAGGAGAAAAAGCA
• BKK38320 (ywbH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGATGCCTCCCTTAT, downstream forward: _UP4_AAGAGAAAGGAGAAAAAGCA

References

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