SubtiBank SubtiBank
ywbH
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ywbH

holin-like protein
locus
BSU_38320
pI
9.59
mw
14.12 kDa
protein length
128 aa Sequence Blast
gene length
387 bp Sequence Blast
function
unknown
product
holin-like protein
essential
no
synonyms
ipa-23r,cidA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    3,933,209 3,933,595

    Phenotypes of a mutant

  • a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' mutant is delayed in pellicle formation [Pubmed|26060272]
  • a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG] [gene|E5E431AC5A7D03EEFF6D694CB25EED1570D739A2|yxaK]-[gene|5A43EDB738641C675D4136269F3638F9B609F238|yxaC] [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]'' triple mutant is severely delayed in pellicle formation [Pubmed|26060272]
  • a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' overexpression strain has more robust pellicle formation [Pubmed|26060272]

    • The protein

    Protein family

  • CidA/LrgA family (with [protein|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|PftA], according to UniProt)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|YwbI]: activation, [Pubmed|26060272], in [regulon|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|YwbI regulon]

    • regulation

  • induced by acetate ([protein|search|YwbI]) [Pubmed|26060272]
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    Biological materials

    Mutant

  • MGNA-B225 (ywbH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1224 NBRP B. subtilis, Japan]
  • BKE38320 ([gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCTCCCTTAT, downstream forward: _UP4_AAGAGAAAGGAGAAAAAGCA
  • BKK38320 ([gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCTCCCTTAT, downstream forward: _UP4_AAGAGAAAGGAGAAAAAGCA

    • References

  • 26060272,15659658,17452642