pbuX

xanthine transport in/out via proton symport

Locus: BSU_22060

Isoelectric point: 8.79

Molecular weight: 46.08 kDa

Protein length: 438 aa

Gene length: 1317 bp

Function: xanthine uptake

Product: xanthine permease

Essential: no

Synonyms: ypaQ

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Nucleotide/ nucleoside transporter
• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,318,127 2,319,443

The protein

Protein family

• xanthine/uracil permease family (according to UniProt)
• Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily (according to UniProt)

Paralogous protein(s)

• PucK, PucJ

Structure

• 5I6C (Aspergillus nidulans purine transporter, 27% identity) PubMed

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: xpt-pbuX
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • G-box: termination, riboswitch PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

  Regulation

  • induced in the absence of guanine (G-box) PubMed

Biological materials

Mutant

• BKE22060 (pbuX::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAGCGTTTTGCCGAATCCAT, downstream forward: _UP4_AAAACAGCAGTCTAACTCCG
• BKK22060 (pbuX::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAGCGTTTTGCCGAATCCAT, downstream forward: _UP4_AAAACAGCAGTCTAACTCCG

References

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