pbuX

xanthine transport in/out via proton symport

locus: BSU_22060

pI: 8.79

mw: 46.08 kDa

protein length: 438 aa

gene length: 1317 bp

function: xanthine uptake

product: xanthine permease

essential: no

synonyms: ypaQ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.6|Nucleotide/ nucleoside transporter]
• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,318,127 2,319,443

The protein

Protein family

• [SW|xanthine/uracil permease family] (according to UniProt)
• [SW|Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily] (according to UniProt)

Paralogous protein(s)

• [protein|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|PucK], [protein|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|PucJ]

Structure

• [PDB|5I6C] (Aspergillus nidulans purine transporter, 27% identity) [pubmed|27088252]

[SW|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]-[gene|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX]
  description: [Pubmed|9098051]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9098051], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|G-box|G-box]: termination, [SW|riboswitch] [Pubmed|9098051,12787499], in [regulon|G-box|G-box]
  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, (molecular inducer: PRPP) [Pubmed|11591660], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]

  regulation

  • induced in the absence of guanine ([SW|G-box]) [Pubmed|12787499]

Biological materials

Mutant

• BKE22060 ([gene|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGCGTTTTGCCGAATCCAT, downstream forward: _UP4_AAAACAGCAGTCTAACTCCG
• BKK22060 ([gene|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGCGTTTTGCCGAATCCAT, downstream forward: _UP4_AAAACAGCAGTCTAACTCCG

References

• 12923093,11591660,9098051,11591660,18763711,27088252