ymcB [2019-02-19 19:42:19]

tRNA methylthiotransferase

locus: BSU17010

pI: 6.87

mw: 58.00 kDa

protein length: 509 aa

gene length: 1530 bp

function: tRNA maturation

product: tRNA methylthiotransferase

essential: no

synonyms: ymcB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 1,772,843 1,774,372

The protein

Catalyzed reaction/ biological activity

• modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA [Pubmed|20472640]

Protein family

• MiaB subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|YqeV]

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|4JC0] (from Thermotoga maritima, 33% for aa 70 ... 504) [pubmed|23542644]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]-[gene|EE52DFA35B935E551871D079A9BE877DB2001A3B|ymcA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• GP1109 (spc), available in [SW|Stlke] lab
• BKE17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA
• BKK17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA

References

• 20472640,23542644