spoIIE [2019-03-12 10:38:41]

protein serine phosphatase, septum-associated PP2C, dephosphorylation of SpoIIAA

Locus: BSU00640

Isoelectric point: 6.14

Molecular weight: 91.78 kDa

Protein length: 827 aa

Gene length: 2484 bp

Function: control of SigF activity, required for normal formation of the asymmetric septum

Product: protein serine phosphatase, septum-associated PP2C

Essential: no

E.C.: 3.1.3.16

Synonyms: spoIIH,spoIIK

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein phosphatases
• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Groups of genes → Membrane proteins

Gene

Coordinates: 70,538 73,021

Phenotypes of a mutant

• delay of the formation of polar FtsZ rings during sporulation PubMed

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of SpoIIAA
• stabilization of ftsZ oligomers PubMed

Protein family

• PP2C phosphatase

Domains

• N-terminal trans-membrane domain PubMed
• central oligomerization and FtsZ interaction domain PubMed
• C-terminal PP2C phosphatase domain PubMed

Cofactors

• Mn ²⁺ PubMed

Effectors of protein activity

• degraded by FtsH, oligomerization and polar localization protect SpoIIE from degradation PubMed

Structure

• 5UCG PubMed
• 3T91 (phosphatase domain) PubMed

Localization

• cell membrane PubMed
• localizes to the polar cell division sites where it causes FtsZ to relocate from mid-cell to form polar Z-rings
• forespore PubMed
• localizes to the cell poles (via DivIVA) PubMed
• proper localization and stability depend on SpoIIQ PubMed

Expression and Regulation

Operons

• Genes: spoIIE
  Description: (DBTBS) null

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Genes: spoIIE-yabS-yabT-tilS-hprT-ftsH
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

Biological materials

Mutant

• MGNA-A083 (spoIIE::erm), available at the NBRP B. subtilis, Japan
• BKE00640 (spoIIE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTCTCATCTCCCACC, downstream forward: _UP4_TAACGCTTCCGTATAAATCA
• BKK00640 (spoIIE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTCTCATCTCCCACC, downstream forward: _UP4_TAACGCTTCCGTATAAATCA

Labs working on this gene/protein

• Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage

References

Reviews

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Modeling of SigF activation

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Original Publications

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