ydbM

acyl-CoA dehydrogenase

locus: BSU_04520

pI: 5.72

mw: 41.98 kDa

protein length: 381 aa

gene length: 1146 bp

function: sulphur metabolism

product: acyl-CoA dehydrogenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 505,152 506,297

The protein

Protein family

• [SW|Acyl-CoA dehydrogenase family] (according to UniProt)

[SW|Cofactors]

• FAD (according to UniProt) [Pubmed|21635694]

Structure

• [PDB|3MXL] (from ''Micromonospora carbonacea var. africana'', 30% identity) [Pubmed|20866105]

Expression and Regulation

Operons

• genes: [gene|7B27ADD1CC7A9BCE0F602FA080B5FD0DB66380A0|ydbM]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

  regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

Biological materials

Mutant

• MGNA-C119 (ydbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2117 NBRP B. subtilis, Japan]
• BKE04520 ([gene|7B27ADD1CC7A9BCE0F602FA080B5FD0DB66380A0|ydbM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGGCAGCCCCCCAGTT, downstream forward: _UP4_TAAAAAAAAGAGCCTTACCG
• BKK04520 ([gene|7B27ADD1CC7A9BCE0F602FA080B5FD0DB66380A0|ydbM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGGCAGCCCCCCAGTT, downstream forward: _UP4_TAAAAAAAAGAGCCTTACCG

References

• 16513748,16513748,20866105