mcsB [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

protein arginine kinase, tags proteins for degradation by ClpCP, adaptor protein, modulator of CtsR-dependent repression

Locus: BSU00850

Isoelectric point: 5.00

Molecular weight: 40.00 kDa

Function: control of CtsR activity

Product: protein arginine kinase

Essential: no

Synonyms: yacI

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Heat shock proteins

Categories containing this gene/protein

• protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

• CtsR regulon, SigB regulon, SigF regulon

Gene

Coordinates on the chromosome (coding sequence): 102,484 -> 103,575

The protein

Catalyzed reaction/ biological activity

• phosphorylates and thereby targets non-functional CtsR for degradation by ClpP/ClpC PubMed

Protein family

• ATP:guanido phosphotransferase family (according to Swiss-Prot)

Modification

• autophosphorylation on specific arginine residues PubMed, dephosphorylation by YwlE PubMed
• autophosphorylation stimulates McsB activity as adaptor protein for ClpC PubMed

Interactions

• McsA-McsB-CtsR, McsB-CtsR PubMed, McsA-McsB
• McsB-YwlE PubMed
• McsB-ClpP/ClpC (rapid degradation of phosphorylated McsB) PubMed

Expression and Regulation

Operon

• ctsR-mcsA-mcsB-clpC-radA-disA PubMed

Sigma factor

• SigA PubMed, SigB PubMed1 PubMed2, SigF PubMed

Regulation

• expressed early during sporulation in the forespore (SigF) PubMed
• induced by stress (SigB) PubMed
• induced by heat (CtsR) PubMed

Regulatory mechanism

• CtsR: transcription repression PubMed

Biological materials

Mutant

• mcsB::aphA3 availbale from the Gerth lab
• mcsBC167S::spec available from the Gerth lab
• GP1457 (mcsB::aphA3), available in Jörg Stülke's lab
• BP69 (spc), available in Fabian Commichau's lab

Expression vector

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
• expression of native mcsB in B. subtilis: pBP186 (in pBQ200), available in Fabian Commichau's lab

Antibody

• available in Gerth lab

Labs working on this gene/protein

• Ulf Gerth, Greifswald, Germany
• Fabian Commichau Göttingen, Germany

References

Reviews

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Original Publications

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