long chain acyl-CoA synthetase, involved in surfactin production
function
fatty acid degradation
product
long chain acyl-CoA synthetase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids][SW|Categories] containing this gene/protein
[SW|utilization of lipids]This gene is a member of the following [SW|regulons]
[SW|CcpA regulon], [SW|FadR regulon]The protein
Catalyzed reaction/ biological activity
ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]Protein family
ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)Paralogous protein(s)
[protein|search|LcfB], [protein|search|YhfT], [protein|search|YngI][SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operon
''[protein|search|lcfA]-[protein|search|fadR]-[protein|search|fadB]-[protein|search|etfB]-[protein|search|etfA]'' [Pubmed|17189250][SW|Sigma factor]
[protein|search|SigA] [Pubmed|21398533]Regulation
subject to carbon catabolite repression ([protein|search|CcpA]-[PtsH|search|HPr(Ser-P)] [Pubmed|21398533]induced by long chain acyl-CoA (C14 ... C20) ([protein|search|FadR]) [Pubmed|17189250]Regulatory mechanism
[protein|search|CcpA]-[PtsH|search|HPr(Ser-P)]: transcription repression [Pubmed|21398533][protein|search|FadR]: transcription repression [Pubmed|17189250]Biological materials
Mutant
available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]References
17189250,17919287,20797616,17085570,22900538 
