fbp

fructose-1,6-bisphosphatase, involved in gluconeogenesis

locus: BSU_40190

pI: 5.60

mw: 77.80 kDa

protein length: 671 aa

gene length: 2016 bp

function: gluconeogenesis

product: fructose-1,6-bisphosphatase

essential: no

ec: 3.1.3.11

synonyms: fdp,yydE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]

Gene

Coordinates: 4,128,029 4,130,044

The protein

Catalyzed reaction/ biological activity

• β-D-fructose 1,6-bisphosphate + H₂O --> β-D-fructose 6-phosphate + phosphate (according to UniProt)

Protein family

• FBPase class 3 family (single member, according to UniProt)

Expression and Regulation

Operons

• genes: [gene|79DD9B2E2EBB4646327AFC2148CE040823DE89D3|fbp]
  description: [pubmed|22383849,9696785]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|9696785], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B811 (yydE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1810 NBRP B. subtilis, Japan]
• BKE40190 ([gene|79DD9B2E2EBB4646327AFC2148CE040823DE89D3|fbp]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACCGTCCATCCCCTTT, downstream forward: _UP4_TGAGGGGTGTGGGGTTCAGG
• BKK40190 ([gene|79DD9B2E2EBB4646327AFC2148CE040823DE89D3|fbp]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACCGTCCATCCCCTTT, downstream forward: _UP4_TGAGGGGTGTGGGGTTCAGG

References

• 6257649,221467,9696785,24571712