nrgA [2019-02-24 18:07:07]

ammonium transporter, required at low ammonium concentration

locus: BSU36510

pI: 5.73

mw: 42.57 kDa

protein length: 404 aa

gene length: 1215 bp

function: ammonium uptake

product: ammonium transporter

essential: no

synonyms: amtB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.8|Uptake of other small ions]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,756,790 3,758,004

The protein

Structure

• [PDB|4NH2] (from ''E. coli'') [Pubmed|17220269]

[SW|Localization]

• cell membrane (according to Swiss-Prot), membrane

Expression and Regulation

Operons

• genes: [gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]
  description: [Pubmed|8282685]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8282685], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|8799114], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • induced by ammonium limitation

Biological materials

Mutant

• GP739 (cat), GP255 (''[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]'', cat), both available in [SW|Jörg Stülke]'s lab [Pubmed|14600241]
• 1A920 ( ''nrgA''::''erm''), [Pubmed|8282685], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A920&Search=1A920 BGSC]
• BKE36510 ([gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT, downstream forward: _UP4_GATTCTATGTGAGGAGTGAC
• BKK36510 ([gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT, downstream forward: _UP4_GATTCTATGTGAGGAGTGAC

lacZ fusion

• pGP168 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab [Pubmed|14600241]

labs

• [SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

References

Reviews

• 10637624,17368911

Original Publications

• 12823818,17001076,8799114,14600241,8282685,1670935,17220269,25229891,25755103