htrB

membrane-anchored protein quality control protease, serine protease, response to secretion and heat stresses

Locus: BSU_33000

Isoelectric point: 4.63

Molecular weight: 48.55 kDa

Protein length: 458 aa

Gene length: 1377 bp

Function: protein quality control

Product: serine protease

Essential: no

E.C.: 3.4.21.107

Synonyms: yvtA,yvtB

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Proteolysis → Protein quality control
• Lifestyles → Coping with stress → Heat shock proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,384,070 3,385,446

The protein

Catalyzed reaction/ biological activity

• Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val (according to UniProt)

Protein family

• Peptidase S1C family (with HtrA and HtrC, according to UniProt)

Domains

• cytoplasmic domain (aa 1 - 71) (according to UniProt)
• transmembrane helix (aa 72 - 92) (according to UniProt)
• extracellular domain (aa 93 - 458) (according to UniProt)
• PDZ domain (aa 350- 449) (according to UniProt)

Structure

• 3QO6 (from Arabidopsis thaliana, 38% identity) PubMed

Localization

• cell membrane (according to UniProt)
• randomly distributed in foci throughout the cell surface PubMed

Expression and Regulation

Operons

• Genes: htrB
  Description: PubMed

  Regulatory mechanism

  • CssR: activation, PubMed, in CssR regulon

  Regulation

  • expressed under conditions of secretion stress (CssR) PubMed

  Additional information

  • HtrB is subject to degradation by WprA and other extracellular proteases PubMed

Biological materials

Mutant

• MGNA-B207 (yvtA::erm), available at the NBRP B. subtilis, Japan
• BKE33000 (htrB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTTACACTCCTTTA, downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG
• BKK33000 (htrB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTTACACTCCTTTA, downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG

References

Reviews

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Original publications

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