comA [2018-09-21 10:34:05]

two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)

Locus: BSU31680

Isoelectric point: 4.76

Molecular weight: 23.98 kDa

Protein length: 214 aa

Gene length: 642 bp

Function: regulation of genetic competence and quorum sensing

Product: two-component system response regulator

Essential: no

E.C.: 4.4.1.19

Synonyms: srfB,comAA

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → Genetic competence
• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Information processing → Regulation of gene expression → Quorum sensing
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 3,252,804 → 3,253,448

The protein

Protein family

• ComA family (according to Swiss-Prot)

Modification

• phosphorylated by ComP on an Asp residue

Effectors of protein activity

• phosphorylation activates DNA binding
• DNA-binding activity of ComA is inhibited by RapC PubMed, RapD PubMed, RapF PubMed, RapH PubMed and RapK PubMed

Structure

• 1U83
• 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: comQ-comX-comP-comA-yuxO
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• BKE31680 (ΔcomA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCCTCCCTTTTAC, downstream forward: _UP4_CTTTAAATGTTGGGGGGTGT
• BKK31680 (ΔcomA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCCTCCCTTTTAC, downstream forward: _UP4_CTTTAAATGTTGGGGGGTGT

References

Reviews

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Original Publications

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