tatAY

component of the [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|TatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|TatCY] twin-arginine translocase

locus: BSU_05980

pI: 6.56

mw: 5.93 kDa

protein length: 57 aa

gene length: 174 bp

function: TAT [SW|protein secretion]

product: component of the twin-arginine translocation pathway

essential: no

synonyms: ydiI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 647,760 647,933

The protein

Catalyzed reaction/ biological activity

• secretion of [protein|3985F88334D7B034A66473C5BB39188AB61A65F6|EfeB] and of [protein|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|YkuE]
• delivers [protein|D4AF96C936093BA44E46FF92A3EDE437495B0923|QcrA] to the membrane [Pubmed|23256564]

Protein family

• TatA/E family (with [protein|8987552EFDF673776741405F7F5FE004556484CA|TatAC] and [protein|E4395255CD43ACB611E0BA872182DF801662C366|TatAD], according to UniProt)

Paralogous protein(s)

• [protein|8987552EFDF673776741405F7F5FE004556484CA|TatAC], [protein|E4395255CD43ACB611E0BA872182DF801662C366|TatAD]

Structure

• [PDB|2L16] ([protein|E4395255CD43ACB611E0BA872182DF801662C366|TatAD], 60% identity) [pubmed|20726548]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Additional information

• [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|TatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|TatCY]-dependent export of [protein|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|YkuE] and [protein|3985F88334D7B034A66473C5BB39188AB61A65F6|EfeB] requires a functional [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|WprA] [Pubmed|23180473]

Expression and Regulation

Operons

• genes: [gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]
  description: [Pubmed|22383849]

  regulation

  • constitutitvely expressed [Pubmed|22383849]

• genes: [gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]-[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]-[gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]
  description: [Pubmed|22383849]

  regulation

  • constitutitvely expressed [Pubmed|22383849]
  • the mRNA is processed between [gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex] and [gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Mutant

• MGNA-C202 (ydiI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2200 NBRP B. subtilis, Japan]
• BKE05980 ([gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTGGGCTCCTCCTTT, downstream forward: _UP4_AAGAAAGAAGATCAGTAAGT
• BKK05980 ([gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTGGGCTCCTCCTTT, downstream forward: _UP4_AAGAAAGAAGATCAGTAAGT

labs

• [SW|Jan Maarten van Dijl], University of Groningen, The Netherlands, [https://www.rug.nl/staff/j.m.van.dijl/ Homepage]

References

Reviews

• 22683878,24140208,25975269,25494301,27121927,31401776

Original publications

• 15554971,19383693,21479178,22041895,22767609,22383849,23180473,23256564,24652282,24875903,26239117,29794222,20726548,32302670