gidA [2018-09-27 14:02:39]

glucose-inhibited division protein, tRNA uridine 5-carboxymethylaminomethyl modification enzyme

Locus: BSU41010

Isoelectric point: 6.33

Molecular weight: 69.58 kDa

Protein length: 628 aa

Gene length: 1884 bp

Function: tRNA modification

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme

Essential: no

Synonyms: mnmG

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 4,209,603 → 4,211,489

Phenotypes of a mutant

• inactivation of gidA reduces sporulation efficiency to 23.9% that of wild type cells; longer mother cells, with some aberrant septa and forespores PubMed

The protein

Protein family

• mnmG family (according to Swiss-Prot)

Structure

• 3G05 (the protein of E. coli, 51% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: thdF-gidA-gidB-noc
  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DnaA: repression, PubMed, in DnaA regulon

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-B876 (gidA::erm), available at the NBRP B. subtilis, Japan
• BKE41010 (ΔgidA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTCTAGTTCCTCCTT, downstream forward: _UP4_ATAGCCGAGTAGAAAGGATG
• BKK41010 (ΔgidA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTCTAGTTCCTCCTT, downstream forward: _UP4_ATAGCCGAGTAGAAAGGATG

References

Reviews

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Original publications

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