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pxpA [2017-8-11 16:0:3]
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pxpA [2017-8-11 16:0:3]

subunit of ATP-dependent 5-oxoprolinase
Locus
BSU04050
Isoelectric point
5.58
Molecular weight
27.39 kDa
Protein length
257 aa Sequence Blast
Gene length
771 bp Sequence Blast
Function
detoxification of 5-oxoproline
Product
subunit of 5-oxoprolinase
Essential
no
Synonyms
ycsF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Detoxification reactions

    • Gene

    Coordinates
    457,023 → 457,796

    Phenotypes of a mutant

  • no growth with 5-oxoproline as single source of nitrogen PubMed
  • reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • 5-oxoproline + ATP --> glutamate + ADP + Pi PubMed

    • Protein family

  • UPF0271 (lamB) family (according to Swiss-Prot)

    • Kinetic information

  • Km (5-oxoproline): 39 µM PubMed

    • Structure

  • 1V6T (from Pyrococcus horikoshii, 52% identity)

    • Expression and Regulation

    Operons

    Genes
    pxpA-ycsG-ycsI-pxpB-pxpC-kipR-lipC
    Description
    PubMed

    Regulatory mechanism

  • KipR: repression, PubMed, in KipR regulon
  • TnrA: activation, PubMed, in TnrA regulon

    • Regulation

  • induced in the presence of 5-oxoproline PubMed
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    Biological materials

    Mutant

  • MGNA-C072 (ycsF::erm), available at the NBRP B. subtilis, Japan

    • References

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