pxpA

subunit of ATP-dependent 5-oxoprolinase

locus: BSU_04050

pI: 5.58

mw: 27.39 kDa

protein length: 257 aa

gene length: 774 bp

function: detoxification of 5-oxoproline

product: subunit of 5-oxoprolinase

essential: no

synonyms: ycsF

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]

Gene

Coordinates: 457,023 457,796

Phenotypes of a mutant

• no growth with 5-oxoproline as single source of nitrogen [pubmed|28830929]
• reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline [pubmed|28830929]

The protein

Catalyzed reaction/ biological activity

• 5-oxoproline + ATP --> glutamate + ADP + P_i [pubmed|28830929]

Protein family

• LamB/PxpA family (single member, according to UniProt)

Kinetic information

• K_m (5-oxoproline): 39 M [pubmed|28830929]

Structure

• [PDB|1V6T] (from ''Pyrococcus horikoshii'', 52% identity)

Expression and Regulation

Operons

• genes: [gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]-[gene|9AD419E3CB86C54A0F11E96C82E80A357C6B23D2|ycsG]-[gene|312E2A4ADFBCB9DD9AD9EEEC9FE53F3932AF5DBA|ycsI]-[gene|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB]-[gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]-[gene|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]-[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]
  description: [Pubmed|9334321]

  regulatory mechanism

  • [protein|7DA9A79876C546B78B716A64706A3A3716018C2E|KipR]: repression, [Pubmed|9334321], in [regulon|7DA9A79876C546B78B716A64706A3A3716018C2E|KipR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|9334321], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • induced in the presence of 5-oxoproline [pubmed|28830929]

Biological materials

Mutant

• MGNA-C072 (ycsF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2070 NBRP B. subtilis, Japan]
• BKE04050 ([gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTGCCAA, downstream forward: _UP4_TCAACATAAAGGAGGAACAA
• BKK04050 ([gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTGCCAA, downstream forward: _UP4_TCAACATAAAGGAGGAACAA

References

• 9334321,25755103,28830929