ykfB [2019-06-19 11:17:39]

L-Ala-D/L-Glu epimerase

locus: BSU12980

pI: 5.91

mw: 39.31 kDa

protein length: 366 aa

gene length: 1101 bp

function: cell wall metabolism

product: L-Ala-D/L-Glu epimerase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]

Gene

Coordinates: 1,366,844 1,367,944

The protein

Structure

• [PDB|1TKK] (complex with substrate), [PDB|1JPM]

Expression and Regulation

Operons

• genes: [gene|9E922F0006DF9E84A8D5B51E9FC35B669872FF90|dppA]-[gene|062AE5FB4C778178A7F6833CB8BD9D631E461E89|dppB]-[gene|EDF8D2C49FA5552B24135E12524F9035E1E01473|dppC]-[gene|7FA72D949B930A3C7FF0391CC6CCC58EEFCA839A|dppD]-[gene|853C570CFB9382449A20BE77B44B4FE44972AA59|dppE]-[gene|C4F85F85F0C99A59145961E34B19D3AD03D2BC22|ykfA]-[gene|77EFC47449D8CC17651ED61C41AC605FCEF01270|ykfB]-[gene|D40ECAE51D9E4791C6FAAA25E2E0D0591D9FAA55|ykfC]-[gene|BB94F5388A0D1FF025E5A88A7E10AE7E4C489E5F|ykfD]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

Biological materials

Mutant

• MGNA-A741 (ykfB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/741 NBRP B. subtilis, Japan]
• BKE12980 ([gene|77EFC47449D8CC17651ED61C41AC605FCEF01270|ykfB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTGTTTCGATTCGAATGA, downstream forward: _UP4_CTTTTGAAAGGGGAAAAAGA
• BKK12980 ([gene|77EFC47449D8CC17651ED61C41AC605FCEF01270|ykfB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTGTTTCGATTCGAATGA, downstream forward: _UP4_CTTTTGAAAGGGGAAAAAGA

References

• 12618455,11747447,11747448,15301535,22392983