skfF [2020-04-06 16:32:31]

ABC transporter (permease), export of the spore killing factor

locus: BSU_01960

pI: 9.22

mw: 51.72 kDa

protein length: 448 aa

gene length: 1344 bp

function: export of the spore killing factor SkfA

product: ABC transporter (permease)

essential: no

synonyms: ybdB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 217,697 → 219,040

Phenotypes of a mutant

• inactivation of ''[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]'' reduces sporulation efficiency to 62.3% that of wild type cells [Pubmed|26735940]

The protein

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]
  description: [Pubmed|16816204]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16452424], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|14651647,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|15687200,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16816204], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]

  additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|SkfA]'-specific transcript, and a weaker 6.5 kb [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH] transcript

Biological materials

Mutant

• MGNA-C516 (ybdB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2514 NBRP B. subtilis, Japan]
• BKE01960 (Δ[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTTTCCTGACCTT, downstream forward: _UP4_TAGTGGTTATGTAGAGTTAT
• BKK01960 (Δ[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTTTCCTGACCTT, downstream forward: _UP4_TAGTGGTTATGTAGAGTTAT

References

Reviews

• 20955377

Original Publications

• 10092453,12817086,16816204,14651647,15687200,15687200,17720793,11731129,17449691,18840696,26735940