skfF

ABC transporter (permease), export of the spore killing factor

Locus: BSU_01960

Isoelectric point: 9.22

Molecular weight: 51.72 kDa

Protein length: 448 aa

Gene length: 1344 bp

Function: export of the spore killing factor SkfA

Product: ABC transporter (permease)

Essential: no

Synonyms: ybdB

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Exporters → Export of peptides
• Lifestyles → Coping with stress → Toxins, antitoxins and immunity against toxins
• Prophages and mobile genetic elements → Prophages → Prophage 1
• Groups of genes → Membrane proteins

Gene

Coordinates: 217,697 → 219,040

Phenotypes of a mutant

• inactivation of skfF reduces sporulation efficiency to 62.3% that of wild type cells PubMed

The protein

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: skfA-skfB-skfC-skfE-skfF-skfG-skfH
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • PhoP: activation, PubMed, in PhoP regulon

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb 'SkfA'-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript

Biological materials

Mutant

• MGNA-C516 (ybdB::erm), available at the NBRP B. subtilis, Japan
• BKE01960 (ΔskfF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTTTCCTGACCTT, downstream forward: _UP4_TAGTGGTTATGTAGAGTTAT
• BKK01960 (ΔskfF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTTTCCTGACCTT, downstream forward: _UP4_TAGTGGTTATGTAGAGTTAT

References

Reviews

Loading

Original Publications

Loading