SubtiBank SubtiBank
yaaH [2019-03-30 18:42:02]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yaaH [2019-03-30 18:42:02]

general stress protein, survival of ethanol stress, SafA-dependent protein of the inner spore coat, spore cortex lytic protein
Locus
BSU00160
Isoelectric point
5.62
Molecular weight
48.47 kDa
Protein length
427 aa Sequence Blast
Gene length
1284 bp Sequence Blast
Function
survival of ethanol stress, protection of the spore
Product
N-acetylglucosaminidase
Essential
no
Synonyms
sleL

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
23,868 25,151

The protein

Protein family

  • Glycosyl hydrolase 18 family (according to UniProt)
  • Paralogous protein(s)

    Domains

  • contains two N-acetylglucosamine-polymer-binding LysM domains at the N-terminus PubMed
  • glycoside hydrolase family 18 domain (aa 125 to 410)
  • Structure

  • 4S3K (SleL from B. megaterium, 55% identity) PubMed
  • Localization

  • inner spore coat, localization depends on SafA PubMed
  • Additional information

  • required for survival of ethanol stress PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigB: sigma factor, PubMed, in SigB regulon
  • Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon
  • Regulation

  • expressed during sporulation in the mother cell (SigE, SpoIIID) PubMed
  • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
  • view in new tab

    Genes
    Description

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon
  • Regulation

  • induced by stress (SigB) PubMed
  • view in new tab

    Biological materials

    Mutant

  • MGNA-B889 (yaaH::erm), available at the NBRP B. subtilis, Japan
  • BKE00160 (yaaH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATAAATTTGAATGAAAA, downstream forward: _UP4_TAATTTGGAAACGTCTTTTT
  • BKK00160 (yaaH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATAAATTTGAATGAAAA, downstream forward: _UP4_TAATTTGGAAACGTCTTTTT
  • References

    Reviews

    Loading

    Original publications

    Loading