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aroD [2019-07-31 12:16:59]
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aroD [2019-07-31 12:16:59]

shikimate dehydrogenase
Locus
BSU_25660
Isoelectric point
6.08
Molecular weight
30.49 kDa
Protein length
280 aa Sequence Blast
Gene length
843 bp Sequence Blast
Function
biosynthesis of aromatic amino acids
Product
shikimate dehydrogenase
Essential
no
E.C.
1.1.1.25
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids

    • Gene

    Coordinates
    2,644,630 2,645,472

    Phenotypes of a mutant

  • slow growth PubMed
  • 50% reduction in mo5U tRNA levels PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Shikimate + NADP+ = 3-dehydroshikimate + NADPH (according to Swiss-Prot)

    • Protein family

  • shikimate dehydrogenase family (single member, according to UniProt)

    • Structure

  • 2HK8 (from Aquifex aeolicus, 42% identity, 61% similarity) PubMed

    • Expression and Regulation

    Operons

    Genes
    yqeG-yqeH-aroD-yqeI-nadD-yqeK-rsfS-yqeM
    Description
    PubMed
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    Biological materials

    Mutant

  • BKE25660 (aroD::erm trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
  • BKK25660 (aroD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC

    • References

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